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igg 14506  (Millipore)

 
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    Millipore igg 14506
    Igg 14506, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg 14506/product/Millipore
    Average 90 stars, based on 1 article reviews
    igg 14506 - by Bioz Stars, 2026-03
    90/100 stars

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    Millipore human serum igg (#14506)
    Mouse monocyte/macrophage (RAW 264.7) cellular uptake of Cy3-labeled human <t>IgG</t> conjugated asymmetric immunoliposomal nanoparticles. Left image shows the placement of the cells. Right image shows the internalized Cy3-labeled <t>immunoliposomes.</t> <t>FITC-labeled</t> immunoliposomes (not shown) were not visible, demonstrating immunoliposomes are internal to the cells. Cells were incubated at 37° C and 5% CO2 in absence of FBS with immunoliposomes 24 h prior to imaging.
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    Image Search Results


    Mouse monocyte/macrophage (RAW 264.7) cellular uptake of Cy3-labeled human IgG conjugated asymmetric immunoliposomal nanoparticles. Left image shows the placement of the cells. Right image shows the internalized Cy3-labeled immunoliposomes. FITC-labeled immunoliposomes (not shown) were not visible, demonstrating immunoliposomes are internal to the cells. Cells were incubated at 37° C and 5% CO2 in absence of FBS with immunoliposomes 24 h prior to imaging.

    Journal: Journal of microencapsulation

    Article Title: Evaluation of Asymmetric Immunoliposomal Nanoparticles for Cellular Uptake

    doi: 10.3109/02652048.2012.696152

    Figure Lengend Snippet: Mouse monocyte/macrophage (RAW 264.7) cellular uptake of Cy3-labeled human IgG conjugated asymmetric immunoliposomal nanoparticles. Left image shows the placement of the cells. Right image shows the internalized Cy3-labeled immunoliposomes. FITC-labeled immunoliposomes (not shown) were not visible, demonstrating immunoliposomes are internal to the cells. Cells were incubated at 37° C and 5% CO2 in absence of FBS with immunoliposomes 24 h prior to imaging.

    Article Snippet: Human serum IgG (#14506) was from Sigma-Aldrich, rabbit glypican-3 antibody (#sc-11395) and FITC labeled goat anti-rabbit IgG (#sc-2012) was from Santa Cruz Biotechnology, and Cy3 labeled goat anti-human IgG (#109095003) was obtained from Jackson Immunology.

    Techniques: Labeling, Incubation, Imaging

    HepG2 liver cancer cell uptake of asymmetric liposomes/immunoliposomes. Top row shows uptake of anti-glypican-3 immunoliposomes. Middle row shows uptake of anti-human IgG immunoliposomes. Bottom row shows uptake of non-antibody conjugated asymmetric liposomes (containing 1 mol% unconjugated DSPE-PEG(2000)-MAL). Cells were incubated overnight with FBS and antibiotic free media in the presence of asymmetric liposomes/immunoliposomes encapsulating Alexa Fluor 555 hydrazide salt. Cells were rinsed and stained with Hoechst 33342 to illuminate the nuclei and FITC-tagged detection antibodies complementary to glypican-3 and human IgG to illuminate non-internalized immunoliposomes. Any fluorescence crossover between the images was systematically removed.

    Journal: Journal of microencapsulation

    Article Title: Evaluation of Asymmetric Immunoliposomal Nanoparticles for Cellular Uptake

    doi: 10.3109/02652048.2012.696152

    Figure Lengend Snippet: HepG2 liver cancer cell uptake of asymmetric liposomes/immunoliposomes. Top row shows uptake of anti-glypican-3 immunoliposomes. Middle row shows uptake of anti-human IgG immunoliposomes. Bottom row shows uptake of non-antibody conjugated asymmetric liposomes (containing 1 mol% unconjugated DSPE-PEG(2000)-MAL). Cells were incubated overnight with FBS and antibiotic free media in the presence of asymmetric liposomes/immunoliposomes encapsulating Alexa Fluor 555 hydrazide salt. Cells were rinsed and stained with Hoechst 33342 to illuminate the nuclei and FITC-tagged detection antibodies complementary to glypican-3 and human IgG to illuminate non-internalized immunoliposomes. Any fluorescence crossover between the images was systematically removed.

    Article Snippet: Human serum IgG (#14506) was from Sigma-Aldrich, rabbit glypican-3 antibody (#sc-11395) and FITC labeled goat anti-rabbit IgG (#sc-2012) was from Santa Cruz Biotechnology, and Cy3 labeled goat anti-human IgG (#109095003) was obtained from Jackson Immunology.

    Techniques: Incubation, Staining, Fluorescence

    (a) HepG2 liver cancer cell uptake of PEGylated asymmetric anti-glypican-3 immunoliposomes. (b) CV-1 monkey kidney cell uptake of PEGylated asymmetric anti-glypican-3 immunoliposomes. In both cases, cells were incubated overnight with FBS and antibiotic free media in the presence of asymmetric immunoliposomes encapsulating Alexa Fluor 555 hydrazide salt and eGFP plasmid. Cells were rinsed and stained with Hoechst 33342 to illuminate the nuclei and FITC-tagged detection antibodies complementary to anti-glypican-3 and human IgG to illuminate non-internalized immunoliposomes. Images were taken 5 days after immunoliposome addition. Any fluorescence crossover between the images was systematically removed. HepG2 cells show internalization of liposomes, however eGFP expression doesn’t occur. Image of External IgG-conjugated liposomes not shown.

    Journal: Journal of microencapsulation

    Article Title: Evaluation of Asymmetric Immunoliposomal Nanoparticles for Cellular Uptake

    doi: 10.3109/02652048.2012.696152

    Figure Lengend Snippet: (a) HepG2 liver cancer cell uptake of PEGylated asymmetric anti-glypican-3 immunoliposomes. (b) CV-1 monkey kidney cell uptake of PEGylated asymmetric anti-glypican-3 immunoliposomes. In both cases, cells were incubated overnight with FBS and antibiotic free media in the presence of asymmetric immunoliposomes encapsulating Alexa Fluor 555 hydrazide salt and eGFP plasmid. Cells were rinsed and stained with Hoechst 33342 to illuminate the nuclei and FITC-tagged detection antibodies complementary to anti-glypican-3 and human IgG to illuminate non-internalized immunoliposomes. Images were taken 5 days after immunoliposome addition. Any fluorescence crossover between the images was systematically removed. HepG2 cells show internalization of liposomes, however eGFP expression doesn’t occur. Image of External IgG-conjugated liposomes not shown.

    Article Snippet: Human serum IgG (#14506) was from Sigma-Aldrich, rabbit glypican-3 antibody (#sc-11395) and FITC labeled goat anti-rabbit IgG (#sc-2012) was from Santa Cruz Biotechnology, and Cy3 labeled goat anti-human IgG (#109095003) was obtained from Jackson Immunology.

    Techniques: Incubation, Plasmid Preparation, Staining, Fluorescence, Expressing